Description
GenJet™ DNA In Vitro Tranfection Reagent for B16-F10 cell is pre-optimized for transfecting B16-F10 cells. B16-F10 is a derived murine melanoma cell line, which is well-established model for the study of experimental cancer therapies. Melanomas express different tumor-associated antigens, which are potential targets for novel designs of therapeutic cancer vaccines. Melanoma-associated antigens include tyrosinase and tyrosine-related protein (TRP)-1/2 or the MAGE and BAGE class o...
Description
GenJet™ DNA In Vitro Tranfection Reagent for SKOV3 cell is pre-optimized for transfecting SKOV3 cells.
Refer to the following optimal transfection conditions for maximal transfection efficiency on SKOV3 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
90%
Cell culture conditions
DMEM with 4.5 g/L glucose...
Description
GenJet™ DNA In Vitro Tranfection Reagent for PC-3 is pre-optimized for transfecting PC-3 cells. This human prostate cancer cell line very sensitive to liposome based mediated toxicity, leading high percentage of cell death. With the pre-optimized DNA transfection reagent, up to 60% efficiency can be achieved.
Refer to the following optimal transfection conditions for maximal transfection efficiency on PC-3 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfecti...
Description
GenJet™ DNA In Vitro Tranfection Reagent for Ca Ski Cell is pre-optimized for transfecting Ca Ski Cells, human cervix epidermoid carcinoma cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on Ca Ski cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
80%
Cell c...
Description
GenJet™ DNA In Vitro Tranfection Reagent for MDCK cell is pre-optimized for transfecting MDCK cells. MDCK cell line is derived from the kidney of an apparently normal, adult, female cocker spaniel; used in numerous viral studies; susceptible to vesicular stomatitis, vaccinia, and Coxsackie viruses, and certain reoviruses and adenoviruses.
The “Shaved Cell Transfection” protocol was optimized to achieve maximal transfection efficiency. Refer to the following optimal t...
Description
GenJet™ DNA In Vitro Tranfection Reagent for MEFs is pre-optimized and conditioned for transfecting Mouse Embryonic Fibroblasts (MEFs). MEFs are required to support the growth of undifferentiated mouse or human ES cells, iPS cells, thus they are also called feeder cells. MEFs are isolated from mouse embryos and are used at their early passages. Before MEFs are used as feeder cells, they must be treated by irradiation or mitomycin C to stop the cells from further dividing. How...
Description
GenJet™ DNA In Vitro Tranfection Reagent for M-PAC is pre-optimized for transfecting M-PAC (also celled mPAC) cells. M-PAC is moderately impregnated pancreas acinar unit cells, also one of hard-to-transfect cell line.
Refer to the following optimal transfection conditions for maximal transfection efficiency on M-PAC cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
Summary of Optimal Transfec...
Description
GenJet™ DNA In Vitro Tranfection Reagent for C6 Glioma cell is pre-optimized for transfecting C6 glioma cells. The C6 glioma cell line is a widely used cell line in neurobiological research. Compared to primary neural cells, the C6 cell line offers some major advantages like easy accessibility and culture as well as a good availability of high cell numbers. Nevertheless, the biggest disadvantage of the C6 cells is that they are difficult-to-transfect with commonly used non-viral ...
Description
GenJet™ DNA In Vitro Tranfection Reagent for SHEP is pre-optimized for transfecting SHEP cells. This human neuroblastoma cell line was cloned from a single primary tumor. Unlike other neuroblastoma cell, SHEP cells does not produce IGF-II, expresses five-fold fewer IGF-IRs than SH-SY5Y and dies in serum-free media or following exposure to metabolic stressors.
Refer to the following optimal transfection conditions for maximal transfection efficiency on SHEP cells. GenJet™ rea...
Description
GenJet™ DNA In Vitro Tranfection Reagent for D 407 is pre-optimized for transfecting D 407, retinal pigment epithelial cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on D 407 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
80%
Cell culture conditions
DMEM...
