Description
GenJet™ DNA In Vitro
Tranfection Reagent for NIH3T3 Cells is pre-optimized
for NIH3T3 cell transfection.
NIH 3T3 cells come from a cell line established in 1962 by two scientists then
at the Department of Pathology in the New York University School of Medicine,
George Todaro and Howard Green. The 3T3 cell line has become the standard
fibroblast cell line. Todaro and Green originally obtained their 3T3 cells from
Swiss mouse embryo tissue.
Refer to the following optimal transfecti...
Description
GenJet™ DNA In Vitro Tranfection Reagent for HuH-7 Cells is pre-optimized for human HuH-7 cell transfection. HuH-7, derived from human cancer liver, is very hard to transfect. With our pripariotary peptide conjugation technology, this reagent was formulated to transfect HuH-7 cells with up to 70% efficiency (GFP positive cells).
Refer to the following optimal transfection conditions for maximal transfection efficiency on HuH-7 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300...
Contents Per Kit:
● 1×1.0 ml of GenJet™ DNA Transfection Reagent
● 1×8.0 ml (5x ) of GenJet™ DNA Transfection Buffer
Description
GenJet™ DNA In Vitro Tranfection Reagent for HUVEC is pre-optimized for transfecting human umbilical vein endothelial cells (HUVEC). HUVEC is one of hard-to-transfect cell lines partly due to the transfection toxicity related cell death. In combination of our proprietary HapiCell™ Toxicity Removal Cocktail, the pre-optimized transfection reagent gave...
Description
GenJet™ DNA In Vitro Tranfection Reagent for Caco-2 Cell is pre-optimized for transfecting Caco-2 cells. The Caco-2 cell line is an immortalized line of heterogeneous human epithelial colorectal adenocarcinoma cells, developed by the Sloan-Kettering Institute for Cancer Research through research conducted by Dr. Jorgen Fogh. The Caco-2 cell line is widely used with in vitro assays to predict the absorption rate of candidate drug compounds across the intestinal epithelial cell b...
Description
GenJet™ DNA In Vitro Tranfection Reagent for MB231 is pre-optimized for transfecting MDA-MB231 cells. The MDA-MB-231 breast cancer cell line was obtained from a pleural effusion of a 51-year-old white female in October 17, 1973, by Dr. R. Calleau at M. D. Anderson Cancer Center. The patient underwent a mastectomy and radiotherapy prior to the removal of the breast cancer specimen. After 52 passages, the karyotype displayed chromosome numbers ranging between 52 and 62, where the ...
Contents Per Kit:
● 1×1.0 ml of GenJet™ DNA Transfection Reagent
● 1×1.0 ml (x50) of HapiCell™ Transfection Toxicity Removal Cocktail
Description
GenJet™ DNA In Vitro Tranfection Reagent for keratinocytes is pre-optimized for transfecting human or mouse primary or immortalized keratinocytes. Keratinocytes are hard to transfect partly due to the transfection toxicity related cell death. In combination of our proprietary HapiCell™ Toxicity Removal Cocktail, the pre-optimized tra...
Description
GenJet™ DNA In Vitro Tranfection Reagent for CV-1 is pre-optimized for transfecting CV-1 cells. The CV-1 cell line was initiated in March of 1964 with a tissue section excised from the kidney of an adult male African green monkey (Cercopithecus aethiops). The fibroblast line was originally utilized in research focusing on the transformation of the cancer-causing Rous sarcoma virus (RSV), but now is popular as a host for acquired immunodeficiency disease (AIDS) research, as well ...
Description
GenJet™ DNA In Vitro Tranfection Reagent for K562 is pre-optimized for transfecting K562 cells. K562 cells were the first human immortalised myelogenous leukaemia line to be established and are a bcr:abl positive erythroleukamia line derived from a 53 year old female CML patient in blast crisis. The cells are non-adherent and rounded, and bear some proteomic resemblance to both undifferentiated granulocytes and erythrocytes. In culture they exhibit much less clumping than many ot...
Description
GenJet™ DNA In Vitro Tranfection Reagent for BHK-21 cell is pre-optimized for transfecting BHK-21 cells. BHK-21 cells is one of the most commonly used cells for vaccine production. When used for this application, usually a cell strain adapted to grow in suspension is used.
Refer to the following optimal transfection conditions for maximal transfection efficiency on BHK-21 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300...
Description
GenJet™ DNA In Vitro Tranfection Reagent for LNCap is pre-optimized for transfecting LNCap cells. LNCap cells are a cell line of human cells commonly used in the field of oncology. LNCaP cells are androgen-sensitive human prostate adenocarcinoma cells derived from the left supraclavicular lymph node metastasis from a 50-year-old caucasian male in 1977. They are adherent epithelial cells growing in aggregates and as single cells.
Refer to the following optimal transfection conditi...
