Description
GenJet™ DNA In Vitro Tranfection Reagent for C2C12 Cell is pre-optimized for transfecting C2C12 cells. C2C12 cells were originally obtained by Yaffe and Saxel (1977) through selective serial passage of myoblasts cultured from the thigh muscle of C3H mice 70 h after a crush injury. These cells were shown to be capable of differentiation. C2C12 cells are a useful model to study the differentiation of non-muscle cells to skeletal muscle cells (e.g myosin phosphorylation mechanisms) ...
Description
GenJet™ DNA In Vitro
Tranfection Reagent for Hela is pre-optimized for Hela cell transfection.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on Hela cells. GenJet™ reagent, 1.0 ml, is sufficient for 600 to
1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
~80%
Cell culture conditions
DMEM with 4.5 g/L glucose, 10% FBS...
Description
GenJet™ DNA In Vitro
Tranfection Reagent for MCF-7 is pre-optimized for transfecting
MCF-7 cells. MCF-7 is a breast cancer cell line was isolated in 1970 from
a 69-year-old Caucasian woman. This cell line retained several
characteristics of differentiated mammary epithelium including the ability to
process estradiol via cytoplasmic estrogen receptors and the capability of
forming domes. This cell line expresses the oncogene Wnt7B. Tumor necrosis
factor alpha (TNF alpha) inhibits t...
Description
GenJet™ DNA In Vitro Tranfection Reagent for Primary Macrophages is pre-optimized for transfecting primary macrophages. Macrophages are white blood cells within tissues, produced by the differentiation of monocytes. Monocytes and macrophages are phagocytes, acting in both non-specific defense (innate immunity) as well as to help initiate specific defense mechanisms (adaptive immunity) of vertebrate animals. Their role is to phagocytose (engulf and then digest) cellular debris and...
Description
GenJet™ DNA In Vitro
Tranfection Reagent for U2OS cells is pre-optimized for transfecting U2OS cells.
The U2OS cell line, originally known as the 2T line, was cultivated from the
bone tissue of a fifteen-year-old human female suffering from osteosarcoma.
Established in 1964, the original cells were taken from a moderately
differentiated sarcoma of the tibia. U2 OS cells exhibit epithelial
adherent morphology and viruses were not detected in the line during
co-cultivation with WI-3...
Description
GenJet™ DNA In Vitro
Tranfection Reagent for CHO is pre-optimized
for transfecting Chinese Hamster Ovary (CHO) cells.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on CHO cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to
600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
~90%
Cell culture conditions
DMEM with 4.5 ...
Description
GenJet™ DNA In Vitro
Tranfection Reagent for HepG2 Cells is pre-optimized
for HepG2 cell transfection.
HepG2 (Hepatocellular carcinoma, human) is a perpetual add cell line which
was derived from the liver tissue of a 15 year old caucasian male with a well
differentiated hepatocellular carcinoma. These cells are epithelial in
morphology, have a model chromosome number of 55 and are not tumorigenic in nude
mice. The cells secrete a variety of major plasma proteins e.g.. albumin, al...
Description
GenJet™ DNA In Vitro
Tranfection Reagent for COS Cells is pre-optimized
for COS cell transfection.
COS is a cell line often used by biologists when studying the monkey virus SV40.
It is also often used to transfect cells to produce recombinant proteins for
molecular biology, biochemistry, and cell biology experiments. Two forms of COS
cell lines commonly used are COS-1 and COS-7. The COS cell line was
obtained by immortalizing a cell line derived from kidney cells of the African
...
Description
GenJet™ DNA In Vitro Tranfection Reagent for Jurkat cell is pre-optimized for transfecting Jurkat cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on Jurkat cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
~70%
Cell culture conditions
PRMI with 10% FBS
Ge...
