Description
GenJet™ DNA In Vitro
Tranfection Reagent for Hela is pre-optimized for Hela cell transfection.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on Hela cells. GenJet™ reagent, 1.0 ml, is sufficient for 600 to
1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
|
~80% |
| Cell culture conditions |
DMEM with 4.5 g/L glucose, 10% FBS |
| GenJet™ (µl) : DNA (µg) Ratio |
3:1 |
Diluent for DNA and Transfection Reagent
|
Serum-free DMEM with 4.5 g/L glucose
|
| Incubation Time to Form GenJet™/DNA Complex |
15 minutes at RT |
| Presence of Serum/Antibiotics during Transfection |
Yes |
| Change Medium 5 Hours After Transfection |
Yes |
| Maximal Efficiency |
48 hours |
Transfection Results:
Reporter Gene
|
EGFP |
Plasmid
|
pEGFP-N3 (CMV promoter)
|
| Efficiency (GFP %) |
88% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on Hela Cells
GenJet™ reagent is optimized for Hela
cell (ATCC # CCL-2.2). Hela cells
were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by
GenJet™. The efficiency was checked 48 hours post transfection
Data Sheet 
