Description
GenJet™ DNA In Vitro
Tranfection Reagent for U2OS cells is pre-optimized for transfecting U2OS cells.
The U2OS cell line, originally known as the 2T line, was cultivated from the
bone tissue of a fifteen-year-old human female suffering from osteosarcoma.
Established in 1964, the original cells were taken from a moderately
differentiated sarcoma of the tibia. U2 OS cells exhibit epithelial
adherent morphology and viruses were not detected in the line during
co-cultivation with WI-38 cells or in CF tests against SV40, RSV, or
adenoviruses. Cells are positive for insulin-like growth factor I (IGF-I) and
insulin-like growth factor II (IGF II) receptors and express a number of
antigens, including blood type A, Rh+, HLA A2, Aw30, B12, Bw35, and B40(+/-).
Refer to the following optimal transfection conditions for maximal transfection
efficiency on U2 OS cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to
600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
|
60~70% |
| Cell culture conditions |
DMEM with 4.5 g/L glucose, 10% FBS |
| GenJet™ (µl) : DNA (µg) Ratio |
3:1 |
Diluent for DNA and Transfection Reagent
|
Serum-free DMEM with 4.5 g/L glucose
|
| Incubation Time to Form GenJet™/DNA Complex |
15 minutes at RT |
| Presence of Serum/Antibiotics during Transfection |
Yes |
| Change Medium 5 Hours After Transfection |
No |
| Maximal Efficiency |
48 hours |
Transfection Results:
Reporter Gene
|
EGFP |
Plasmid
|
pEGFP-N3 (CMV promoter)
|
| Efficiency (GFP %) |
95% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on U2 OS Cells
GenJet™ reagent is optimized for
U2OS. U2OS cells
were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by
GenJet™. The efficiency was checked 48 hours post transfection
Data Sheet 
