GenJet™ In Vitro DNA Tranfection Reagent for Jurkat Cell

¥ 2,680.00

购买数量：

目录号: SL100489-JURKAT
包装: 1.0 ml

请选择:

包装

Description

GenJet™ DNA In Vitro Tranfection Reagent for Jurkat cell is pre-optimized for transfecting Jurkat cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on Jurkat cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Confluence on the day of transfection	~70%
Cell culture conditions	PRMI with 10% FBS
GenJet™ (µl) : DNA (µg) Ratio	3:1
Diluent for DNA and Transfection Reagent	1xGenJet™ Transfection Buffer
Incubation Time to Form GenJet™/DNA Complex	15 minutes at RT
Presence of Serum/Antibiotics during Transfection	Yes
Change Medium After Transfection	No
Maximal Efficiency	48 hours