Description
GenJet™ DNA In Vitro
Tranfection Reagent for NIH3T3 Cells is pre-optimized
for NIH3T3 cell transfection.
NIH 3T3 cells come from a cell line established in 1962 by two scientists then
at the Department of Pathology in the New York University School of Medicine,
George Todaro and Howard Green. The 3T3 cell line has become the standard
fibroblast cell line. Todaro and Green originally obtained their 3T3 cells from
Swiss mouse embryo tissue.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on NIH 3T3 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to
600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
|
~70% |
| Cell culture conditions |
DMEM with 4.5 g/L glucose, 10% FBS |
| GenJet™ (µl) : DNA (µg) Ratio |
3:1 |
Diluent for DNA and Transfection Reagent
|
Serum-free DMEM with 4.5 g/L glucose
|
| Incubation Time to Form GenJet™/DNA Complex |
15 minutes at RT |
| Presence of Serum/Antibiotics during Transfection |
Yes |
| Change Medium 5 Hours After Transfection |
No |
| Maximal Efficiency |
48 hours |
Transfection Results:
Reporter Gene
|
EGFP |
Plasmid
|
pEGFP-N3 (CMV promoter)
|
| Efficiency (GFP %) |
90% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on NIH 3T3 Cells. NIH 3T3 cells
were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by
GenJet™. The efficiency was checked 24 hours post transfection
Data Sheet 
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