GenJet™ In Vitro DNA Tranfection Kit for HUVEC

¥ 2,880.00

购买数量:

  • 目录号: SL100489-HUVEC
  • 包装: 1.0 ml

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Contents Per Kit:
1×1.0 ml of GenJet™ DNA Transfection Reagent
● 1×8.0 ml (5x ) of
GenJet™ DNA Transfection Buffer

Description

GenJet™ DNA In Vitro Tranfection Reagent for HUV
EC is pre-optimized for transfecting human umbilical vein endothelial cells (HUVEC). HUVEC is one of hard-to-transfect cell lines partly due to the transfection toxicity related cell death. In combination of our proprietary HapiCell™ Toxicity Removal Cocktail, the pre-optimized transfection reagent gave up to 60% efficiency. To achieve maximal efficiency, the “Shaved Cell Transfection” protocol was optimized for transfecting HUVEC. Refer to the following optimal transfection conditions for maximal transfection efficiency on HUVEC. GenJet™ reagent, 1.0 ml, is sufficient for 300 transfections in 24 well plates or 150 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Cell culture medium DMEM with 4.5 g/L glucose, 10% FBS
Confluency of cell on the day of shaving ~90%
Cell density for plating 1.3×10^5 cells per square centimeter
GenJet™ (µl) : DNA (µg) Ratio 2.25:1
Diluent for DNA and Transfection Reagent GenJet™ DNA Transfection Buffer (1x )
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Incubation time of shaved cells with transfection complex 20 minutes at 37 degree
Maximal Efficiency 48 hours

Transfection Results:

Reporter Gene
EGFP
Plasmid
pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 60%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet




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