Contents Per Kit:
● 1×1.0 ml of GenJet™ DNA Transfection Reagent
● 1×8.0 ml (5x ) of GenJet™ DNA Transfection Buffer
Description
GenJet™ DNA In Vitro Tranfection Reagent for HUVEC is pre-optimized for transfecting human umbilical vein endothelial cells (HUVEC). HUVEC is one of hard-to-transfect cell lines partly due to the transfection toxicity related cell death. In combination of our proprietary HapiCell™ Toxicity Removal Cocktail, the pre-optimized transfection reagent gave up to 60% efficiency. To achieve maximal efficiency, the “Shaved Cell Transfection” protocol was optimized for transfecting HUVEC. Refer to the following optimal transfection conditions for maximal transfection efficiency on HUVEC. GenJet™ reagent, 1.0 ml, is sufficient for 300 transfections in 24 well plates or 150 transfections in 6 well plates.
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet 
