GenJet™ In Vitro DNA Tranfection Kit for Keratinocytes

¥ 1,680.00

购买数量:

  • 目录号: SL100489-KERA
  • 包装: 1 kit (2x1.0 ml)

请选择:



Contents Per Kit:
1×1.0 ml of GenJet™ DNA Transfection Reagent
● 1×1.0 ml (x50) of HapiCell™ Transfection Toxicity Removal Cocktail

Description

GenJet™ DNA In Vitro Tranfection Reagent for keratinocytes is pre-optimized for transfecting human or mouse primary or immortalized keratinocytes. Keratinocytes are hard to transfect partly due to the transfection toxicity related cell death. In combination of our proprietary HapiCell™ Toxicity Removal Cocktail, the pre-optimized transfection reagent gave up to 50% efficiency with minimal cell death.

Refer to the following optimal transfection conditions for maximal transfection efficiency on keratinocytes. GenJet™ reagent, 1.0 ml, is sufficient for ~600 transfections in 24 well plates or ~300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Confluence on the day of transfection
90%
Cell culture conditions DMEM with 4.5 g/L glucose, 10% FBS
GenJet™ (µl) : DNA (µg) Ratio 3:1
Diluent for DNA and Transfection Reagent
Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Presence of Serum/Antibiotics during Transfection Yes
Change Medium After Transfection Yes, ~5 Hours After Transfection
Treatment with HapiCell™ Toxicity Removal Cocktail Yes, ~5 Hours After Transfection Followed by PBS Wash
Maximal Efficiency 48 hours

Transfection Results:

Reporter Gene
EGFP
Plasmid
pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 56%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet




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