GenJet™ In Vitro DNA Tranfection Reagent for B16-F10 Cells

¥ 1,560.00

购买数量:

  • 目录号: SL100489-B16
  • 包装: 1.0 ml

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Description

GenJet™ DNA In Vitro Tranfection Reagent for B16-F10 cell is pre-optimized for transfecting B16-F10 cells. B16-F10 is a derived murine melanoma cell line, which is well-established model for the study of experimental cancer therapies. Melanomas express different tumor-associated antigens, which are potential targets for novel designs of therapeutic cancer vaccines. Melanoma-associated antigens include tyrosinase and tyrosine-related protein (TRP)-1/2 or the MAGE and BAGE class of antigens. Many immunotherapeutic protocols have been tested using the murine B16-F10 melanoma cell line that originated in the syngeneic C57BL/6 (H-2b) mouse strain.

Refer to the following optimal transfection conditions for maximal transfection efficiency on B16-F10 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Confluence on the day of transfection
~90%
Cell culture conditions DMEM with 4.5 g/L glucose, 10% FBS
GenJet™ (µl) : DNA (µg) Ratio 3:1
Diluent for DNA and Transfection Reagent
Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Presence of Serum/Antibiotics during Transfection Yes
Change Medium After Transfection Yes, ~5 Hours After Transfection
Maximal Efficiency 48 hours

Transfection Results:

Reporter Gene
EGFP
Plasmid
pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 88%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet




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