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GenJet™ In Vitro DNA Tranfection Reagent for PC-3 Cells

¥ 1,560.00

购买数量：

  • 目录号: SL100489-PC3
  • 包装: 1.0 ml

请选择:



Description

GenJet™ DNA In Vitro Tranfection Reagent for PC-3 is pre-optimized for transfecting PC-3 cells.  This human prostate cancer cell line very sensitive to liposome based mediated toxicity, leading high percentage of cell death.  With the pre-optimized DNA transfection reagent, up to 60% efficiency can be achieved.

Refer to the following optimal transfection conditions for maximal transfection efficiency on PC-3 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Confluence on the day of transfection
 90%
Cell culture conditions DMEM with 4.5 g/L glucose, 10% FBS
GenJet™ (µl) : DNA (µg) Ratio 3:1
Diluent for DNA and Transfection Reagent
 Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Presence of Serum/Antibiotics during Transfection Yes
Change Medium After Transfection Yes, ~5 Hours After Transfection
Maximal Efficiency 48 hours

Transfection Results:

Reporter Gene
 EGFP
Plasmid
 pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 60%

Storage Condition
 Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet




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