GenJet™ In Vitro DNA Tranfection Reagent for MDCK Cells

¥ 1,560.00

购买数量:

  • 目录号: SL100489-MDCK
  • 包装: 1.0 ml

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Description

GenJet™ DNA In Vitro Tranfection Reagent for MDCK cell is pre-optimized for transfecting MDCK cells. MDCK cell line is derived from the kidney of an apparently normal, adult, female cocker spaniel; used in numerous viral studies; susceptible to vesicular stomatitis, vaccinia, and Coxsackie viruses, and certain reoviruses and adenoviruses.

The “Shaved Cell Transfection” protocol was optimized to achieve maximal transfection efficiency. Refer to the following optimal transfection conditions for maximal transfection efficiency on MDCK cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 transfections in 24 well plates or 150 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Cell culture medium DMEM with 4.5 g/L glucose, 10% FBS
Confluency of cell on the day of shaving 95~100%
Cell density for plating 1.3×10^5 cells per square centimeter
GenJet™ (µl) : DNA (µg) Ratio 4:1
Diluent for DNA and Transfection Reagent
Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 12 minutes at RT
Incubation time of shaved cells with transfection complex 20 minutes at 37 degree
Maximal Efficiency 48 hours

Transfection Results:

Reporter Gene
EGFP
Plasmid
pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 70%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer


A Picture Showing Transfection Efficiency of GenJet™ Reagent on MDCK Cells. MDCK cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 24 hours post transfection

Data Sheet




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