Features:
– Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells.
– Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9).
– Integration into chromosome 19 of host genome.
– Induce efficient gene silencing.
– Wide choice of AAV cis vectors (promoterless, CMV, CAG (also known as CBA), U6, H1, UBC, Synapsin promoters with or without reporter genes like GFP, mRFP, Luc, LacZ, etc).
Service Description:
– Medium scale transfection of AAV·HT™ 293 cells into 1xcell stack.
– Harvest rAAV followed by purification via column filtration.
– Titration via qPCR (measuring genome copies).
Required Materials:
AAV cis plasmid carrying your shRNA, >80 µg.
Turnaround Time:
~ 1 week.
Deliverables: >2.0 ml of purified high titer at >1E+12 VG/ml* AAV stock for in vitro application ONLY.
We offer discount for new customer, please request a quote with us today.
We also offer truncated custom shRNA AAV service, please request a quote with us today.
* Final viral yield may depend on the nature of transgene.
