GenJet™ In Vitro DNA Tranfection Reagent for D 407 Cells

¥ 1,560.00

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目录号: SL100489-D407
包装: 1.0 ml

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包装

Description

GenJet™ DNA In Vitro Tranfection Reagent for D 407 is pre-optimized for transfecting D 407, retinal pigment epithelial cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on D 407 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Confluence on the day of transfection	80%
Cell culture conditions	DMEM with 4.5 g/L glucose, 10% FBS
GenJet™ (µl) : DNA (µg) Ratio	3:1
Diluent for DNA and Transfection Reagent	Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex	15 minutes at RT
Presence of Serum/Antibiotics during Transfection	Yes
Change Medium After Transfection	Yes, 18~24 Hours After Transfection
Maximal Efficiency	48 hours