GenJet™ In Vitro DNA Tranfection Reagent for Neuro-2A Cells

¥ 1,560.00

购买数量:

  • 目录号: SL100489-N2A
  • 包装: 1.0 ml

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Description

GenJet™ DNA In Vitro Tranfection Reagent for Neuro2A is pre-optimized for transfecting Neuro2A cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on Neuro2A cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Confluence on the day of transfection
90%
Cell culture conditions DMEM with 4.5 g/L glucose, 10% FBS
GenJet™ (µl) : DNA (µg) Ratio 3:1
Diluent for DNA and Transfection Reagent
Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Presence of Serum/Antibiotics during Transfection Yes
Change Medium 5 Hours After Transfection Yes
Maximal Efficiency 48 hours

Transfection Results:

Reporter Gene
EGFP
Plasmid
pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 89%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer


A Picture Showing Transfection Efficiency of GenJet™ Reagent on Neuro2A Cells.  Neuro2A cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™ and visualized by a Nikon Eclipse Fluorescence microscope with DIC phase (left panel) and FITC (right panel) imaging 36 hours post transfection

Data Sheet




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