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GenJet™ In Vitro DNA Tranfection Reagent for Rin Related Cells

¥ 1,780.00

购买数量：

  • 目录号: SL100489-RIN
  • 包装: 1.0 ml

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Description

GenJet™ DNA In Vitro Tranfection Reagent for Rin is pre-optimized znd conditioned for transfecting Rin and related cells including Rin-14B, Rin-m5F, Rin-m and Rin-5F. Refer to the following optimal transfection conditions for maximal transfection efficiency on Rin related cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Confluence on the day of transfection
 90%
Cell culture conditions DMEM with 4.5 g/L glucose, 10% FBS
Transfection Medium
 DMEM with 4.5 g/L glucose, 1% FBS
GenJet™ (µl) : DNA (µg) Ratio 3:1
Diluent for DNA and Transfection Reagent
 Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Presence of Serum/Antibiotics during Transfection Yes in presence of 1% FBS
Change Medium 5 Hours After Transfection No
Maximal Efficiency 48 hours

Transfection Results:

Reporter Gene
 EGFP
Plasmid
 pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 85%

Storage Condition
 Store at 4 °C. If stored properly, the product is stable for 12 months or longer

A Picture Showing Transfection Efficiency of GenJet™ Reagent on Rin-5F Cells. Rin-5F cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 24 hours post transfection

Data Sheet




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