GenJet™ In Vitro DNA Tranfection Reagent for Epithelial Cell

 Description

GenJet™ DNA In Vitro Tranfection Reagent for epithelial cell is pre-optimized for epithelial cells. By cross-linking a peptide ligand, this pre-optimized GenJet™ reagent specifically binds epithelial cells and give superior efficiency on epithelial cells.

Refer to the following optimal transfection conditions for maximal transfection efficiency on epithelial cells. GenJet™ reagent, 1.0 ml, is sufficient for 600 to 1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates.

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

A comparison showing superior efficiency of the pre-optimized GenJet (Ver. II) on normal human colonic epithelial cells.  Transfection was performed per the vendors’ protocol.  0.5 µg of GFP DNA (pEGFP-N3) per well in 24-well plate was delivered to normal human colonic epithelial cells with different transfection methods.  The GFP positive cells were counted under a Nikon 2000 Eclipse fluorescence microscope 24 hours post transfection.

Data Sheet