Description:
GenMute™ siRNA Transfection Reagent for Hela Cell is pre-optimized for transfecting siRNA to Hela cells. GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 10 pmoles siRNA or miRNA mimics in 24-well plate.
Refer to the following optimal transfection conditions for maximal silencing on Hela cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions:
Cell culture medium
Confluency of cell on the day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute™ Reagent
Incubation Time to Form GenMute™/siRNA Complex
Maximal Silencing Efficiency
Transfection Results:
Reporter Gene
Silencing Efficiency (% ) |
DMEM with 4.5 g/L glucose, 10% FBS
~50%
20.0 nM
1xGenMute™ Transfection Buffer
~15 minutes at RT
48 hours
Renilla Luciferase
90% |
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Data Sheet & Protocol 
