Description:
GenMute™ siRNA Transfection Reagent for RAW 264.7 Cell is pre-optimized for transfecting siRNA to RAW 264.7 cells. RAW 264.7 cells are a macrophage-like, Abelson leukemia virus transformed cell line derived from BALB/c mice. RAW 264.7 cell slide is often used for immunocytochemical analysis of protein and protein-protein interaction. Procedures for immunohistochemistry can be directly applied.
Size & content:
– GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 15 pmoles siRNA or miRNA mimics in 24-well plate
– GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution
Refer to the following optimal transfection conditions for maximal silencing on RAW 264.7 cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Cell culture medium
Confluency of cell on the day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute™ Reagent
Incubation Time to Form GenMute™/siRNA Complex
Maximal Silencing Efficiency
Transfection Results:
Reporter Gene
Silencing Efficiency (% ) |
DMEM with 4.5 g/L glucose, 10% FBS
~50%
30 nM
1xGenMute™ Transfection Buffer
~15 minutes at RT
48 hours
Renilla Luciferase
90% |
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Data Sheet & Protocol 
