Description:
HapiCell™ Transfection Toxicity Removal Cocktail was our proprietary formulation for effectively eliminating DNA or siRNA transfection mediated toxicity. Incubation of sensitive cells (e.g., SaoS-2, HUVEC, primary kerotinocytes, etc) with HapiCell Toxicity Removal Reagent for 2 minutes at RT was confirm to completely remove the transfection related toxicity which otherwise kill the cells. HapiCell™ Transfection Toxicity Removal Cocktail, 125 ml, is sufficient to treat 125 wells for 24-well plate and 63 wells for 6-well plate respectively.
Feature:
– Removes transfection meidated toxicity after 2 minutes incubation with cells at RT
– Remove extracellular complexes of DNA and cationic lipids or polymers
– Nontoxic to cells
– Maintain cell viability
HapiCell™ Transfection Toxicity Removal Cocktail Effectively Remove Transfection Mediated Toxocity:
A example showing complete removal of transfection toxicity after treatment with HapiCell™ Transfection Toxicity Removal Cocktail on mouse skin fibroblast. A DNA encoding GFP was delivered to mouse skin fibroblasts by Lipofectamine 2000 in absence of serum. Serum-containing medium was changed back 5 hours post transfection followed 2 minutes incubation with (right panel) or without (left panel) HapiCell™ Transfection Toxicity Removal Cocktail. The cell viability was checked under microscope 24 hours post transfection
A example showing complete removal of transfection toxicity after treatment with HapiCell™ Transfection Toxicity Removal Cocktail on primary mouse kerotinocytes. A DNA encoding GFP was delivered to primary mouse kerotinocytes by Lipofectamine 2000 in absence of serum. Serum-containing medium was changed back 5 hours post transfection followed 2 minutes incubation with (right panel) or without (left panel) HapiCell™ Transfection Toxicity Removal Cocktail. The cell viability was checked under microscope 24 hours post transfection
Data Sheet 
