Features:
– Nonpathogenic with least immune response.
– Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells.
– Integration into chromosome 19 of host genome.
– Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9).
– Wide choice of AAV cis vectors (promoterless, CMV, CAG (also known as CBA), U6, H1, UBC, Synapsin promoters with or without reporter genes like GFP, mRFP, Luc, LacZ, etc).
Service Description:
– Medium scale transfection of AAV·HT™ 293 cells into 20×150 mm plates.
– Harvest rAAV followed by purification via column filtration.
– Titration via qPCR (measuring genome copies).
Required Materials:
AAV cis plasmid carrying your gene of interest (GOI), >150 µg.
Turnaround Time:
1 ~ 2 weeks.
Deliverables:
>1.0 ml of purified high titer at >1E+12 VG/ml* AAV stock for in vitro application ONLY.
We offer discount for new customer, please request a quote with us today.
* Final viral yield may depend on the nature of transgene.
